Effect of Low-Power DPSS Laser Irradiation upon the Stability of Packed RBCs after Different Storage Periods
Keywords:
Stored RBCS, Low-power DPSS laser, Blood stabilityAbstract
Numerous studies have demonstrated that laser therapy significantly enhances biological systems; low- power lasers have been frequently used in medicinal applications. The aim of the research is to determine whether the stability of stored RBCs is impacted by low-power DPSS laser irradiation. Venipuncture was used to collect human blood samples, which were then put in tubes with the anticoagulant citrate-phosphate dextrose-adenine (CPDA-1). After being separated into eight equal aliquots, the blood sample was kept at 4ºC for 21 days. The stability test was conducted on days 0, 7, 14, and 21 of storage for both radiated and non-irradiated aliquots. The test measures the percentage of hemolysis of an overnight sored RBC in saline solution. The irradiated aliquots were exposed to a DPSS laser at a wavelength of 650 nm with dosages of 30, 50, and 70 J/cm² for 15 minutes. Exposure of RBC suspension to a 650 nm wavelength low-power laser and a radiation doses of 70 J/ cm², 50 J/ cm², and 30 J/ cm² was associated with a significant reduction in the percentage of hemolysis that ranged from 3% to 11% throughout the whole storage time. In conclusion, it is observed that throughout storage periods, the 70 J/ cm² has a greater impact on the stability of the RBC suspensions.
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