Preparation of Water-Insoluble Films Based on Silk Fibroin and Polysaccharide

Abstract

Silk fiber consists of fibroin (72-81%), sericin (19-28%) and other oils and waxes. Bombyx mori fibers consist of at least 16 amino acid residues, Silk has high molecular weight (200-350 kDa or more), large repetitive modular hydrophobic domains interrupted by small hydrophilic groups. Bombyx mori silk fibroin consists of heavy ( H ) and light ( L ) chains ( H – and L – fibroin, respectively ) connected by disulfide bonds. Ajizawa reagent (calcium chloride:ethanol:water (1:8:2) molar ratio solution) was used to dissolve the isolated fibroin in our further studies . Fibroin was dissolved in ajizawa solution (fibroin: ajizawa solution in a ratio of 1:20) at a temperature of 55 o C for 1 hour under constant stirring. Fibrous materials derived from polysaccharides and proteins affect the body's natural environment and thereby provide optimal conditions for tissue growth and regeneration. We obtained fibroin-polysaccharide based composites in our study as well. For this, fibroin and dextran, purified according to the above methods (dialdehyde dextran) composite material in the form of a film was obtained.