Morpho-Immunohistological Analysis the Lower Respiratory System in Adults Rats
Keywords:
Immunohistochemical, Morpho, Adult, RatAbstract
The goal of this research is to achieve a better understanding of the histomorphology of the lower respiratory system in Sprague-Dawley rats. The lower respiratory tract is made up of the trachea, bronchi, bronchioles, and terminal bronchi. Distinct tissues assist with oxygen supply, gas exchange, and immunological defense systems throughout the lower respiratory system. Airways continue to branch while reducing in diameter.
Twenty rats were divided into two groups of ten rats each. One group was used for morphometric measurements, which included tracheal and lung lengths and diameters. The mean values for the first group were 3.5±0.05e and 0.510±0.002e for the trachea, and 5.08±0.06e and 0.501±0.001e for the lung. The second group studied histometric and histochemical sections that had been fixed in 10% formaldehyde, embedded in paraffin wax, sectioned, and stained with Masson's Trichrome and routine stain (H&E). The tracheal wall was made up of mucosa, submucosa, hyaline cartilage, and adventitia. The mucosa was bordered by pseudostratified and ciliated respiratory epithelial cells. This epithelium consisted of basal cells, goblet cells, and ciliate cells. The open end and elastic connective tissues of the C-ring hyaline cartilage also house the smooth trachealis muscle. The thick fibroblastic tissue that surrounds the hyaline cartilage layer contains chondrocytes in the lacuna within an amorphous matrix. Collagen II penetrated the entire cartilage ring, significantly darkening the tracheal hyaline cartilage. The cartilage ring's perimeter contained collagen II.
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