Protective Role of Flavonoid on Histological Architecture of Kidney and Enzymatic Functions of Liver in Heat-Stressed Male Mice

The current study of the evaluation of renal and hepatic function of male mice exposed to the stressed environment condition. In the experiment design, twenty-four male mice have been divided into three groups: the control group drenched normal saline, the stressed group drenched normal saline and kept for 3 weeks, and the flavonoid group drenched flavonoid 25 mg/kg b.w. for 3 weeks. After 24 hours of flavonoid administration, synthesized mice with kitamine were subjected for blood draining to measurement serum MDA, GPx, AST, and ALT as well as to collection the kidney specimens for histology. The result of serum antioxidant enzyme MDA and GPx among groups of experiment showed highly significant effect between stressed group under heat stress and control. The decrement of serum MDA in the flavonoid group showed a significant effect when compared with the stressed group, while there was a slightly significant difference between the control and flavonoid groups showed significant decreased serum GPx of stressed group, while increase in serum GPx of flavonoid group were seen compared to stressed group, and slightly significant between control and flavonoid group. The result of hepatic function referred to effect of stressed on liver significant elevation in serum AST and ALT of stressed in comparison to other groups. Histopathalogical effects of stressed environment on renal tissues showed influx of the inflammatory cells to the renal tissues and epithelial degeneration appears. Also, hemorrhage, edema, fatty degeneration and accumulation of inflammatory cells were observed. Flavonoid administration to the adult male mice showed decreased effects of stressed on renal tissues through reduction inflammatory cells, edema, hemorrhage and epithelial cell degeneration when compared with the stressed group. In conclusion, the experiment referred to the effects of stressed environmental conditions on cellular activity through effects on cellular enzymes, cell membrane regulation, and metabolism.