Evaluation of Serum Toll-Like Receptor 9 (TLR9) Levels Among Iraqi Patients with Type 2 Diabetes Mellitus

Authors

  • Shahad Dyaa Salman Kashmer Department of Biology, College of Education for Pure Sciences, University of Wasit – Iraq
  • Intisar Hussein Ahmed Department of Biology, College of Education for Pure Sciences, University of Wasit – Iraq

Abstract

This study aimed to evaluate serum levels of Toll-like receptor 9 (TLR9) in Iraqi patients with type 2 diabetes mellitus (T2DM) and to compare them with healthy controls. A total of 45 T2DM patients and 35 healthy individuals were enrolled. Serum TLR9 levels were measured using the enzyme-linked immunosorbent assay (ELISA) technique.

The mean TLR9 concentration was significantly lower in T2DM patients (173.75 ± 8.52 ng/mL) compared to healthy controls (270.79 ± 20.61 ng/mL) (P < 0.00001). When analyzed by gender, male diabetic patients had higher levels than females (189.09 ± 11.45 vs. 159.09 ± 12.03 ng/mL, P = 0.0391). However, both sexes in the diabetic group had significantly lower levels compared to the healthy group. No significant difference was observed between males and females in the control group.

These findings suggest that reduced serum TLR9 levels are associated with T2DM and may reflect an immune-inflammatory imbalance that contributes to disease pathogenesis.

References

1. Saeedi, P., et al. (2019). Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045. Diabetes Research and Clinical Practice, 157, 107843.

2. Hemmi, H., et al. (2000). A Toll-like receptor recognizes bacterial DNA. Nature, 408(6813), 740–745.

3. Donath, M. Y., & Shoelson, S. E. (2011). Type 2 diabetes as an inflammatory disease. Nature Reviews Immunology, 11(2), 98–107.

4. Kawai, T., & Akira, S. (2010). The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nature Immunology, 11(5), 373–384.

5. Kumar, H., et al. (2009). Pathogen recognition by the innate immune system. International Reviews of Immunology, 28(3–4), 117–143.

6. Brown, K. L., et al. (2020). Innate immune memory and tissue-specific immunity in metabolic disease. Nature Immunology, 21(10), 1127–1134.

7. Al-Sarray, A.J. and Ahmed, I.H., 2021. Serum TNF-Α Concentrations In Type Two Diabetes Mellitus. NVEO-NATURAL VOLATILES & ESSENTIAL OILS Journal| NVEO, pp.540-546.

8. Ghali,.Z.H.and Jebil,H.D., 2021. Evaluation Of Serum Levels Of Cyclooxygenase-1 (COX-1) Among Patients With Type Two Diabetes Mellitus (T2DM). NVEONATURAL VOLATILES & ESSENTIAL OILS Journal| NVEO, pp.511-515.

9. Hussein, A.R. and Ghali, Z.H., 2022. Analysis Of Cell Free DNA And Cf-Mt DNA As Molecular Markers In Patients With Type-2 Diabetes Mellitus. NVEONATURAL VOLATILES & ESSENTIAL OILS Journal| NVEO, pp.444-449.

10. Yousif, B.A. and Ghali, Z.H., 2021. Evaluation Of Serum Levels Of Interleukin-10 Among Patients With Type Two Diabetes Mellitus (T2DM). NVEO-NATURAL VOLATILES & ESSENTIAL OILS Journal| NVEO, pp.496-499.

11. Mahmood, D.Q. and Ghali, Z.H., 2022. Evaluation of Osteoprotegerin (OPG) levels among Iraqi type2 diabetic patients, 6(S7), 517–521.

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Published

2025-06-23

How to Cite

Kashmer, S. D. S., & Ahmed, I. H. (2025). Evaluation of Serum Toll-Like Receptor 9 (TLR9) Levels Among Iraqi Patients with Type 2 Diabetes Mellitus. International Journal of Pediatrics and Genetics , 3(6), 74–76. Retrieved from https://medicaljournals.eu/index.php/IJPG/article/view/1932