Khudayorov Sardor Juratovich (1)
This article is devoted to the significance of the polymerase chain reaction (PCR) method in modern clinical laboratory diagnostics. It highlights the molecular-biological foundations of PCR technology, the mechanisms of in vitro amplification of DNA and RNA fragments, as well as the diagnostic capabilities of conventional PCR, real-time PCR (qPCR), and reverse transcription PCR (RT-PCR). In addition, the role of PCR in the detection of infectious, hereditary, and oncological diseases, genetic identification of pathogens, assessment of viral load, and monitoring of treatment effectiveness is analyzed. The article briefly reviews pre-analytical and analytical errors encountered in clinical application of PCR, the risk of contamination, and the limitations of the method.This work has scientific and practical significance for specialists and residents working in the field of laboratory medicine.
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