Immune Profiling of Humoral and Inflammatory Responses in Pediatric Immune Thrombocytopenia in Non-Admitted Children Aged 2–10 Years

Introduction: Idiopathic thrombocytopenic purpura (ITP) is a common acquired autoimmune disease of childhood characterized by isolated thrombocytopenia. Ineffective immune control by the humoral and cytotoxic responses may be permissive for disease initiation and progression. In this study we aimed to estimate the CRP, Anti- IgG, IgM- and IgA responses in NonAdmitted pediatric ITP patients vs healthy controls.

Methods: A case–control study was conducted on 20 ITP patients and 20 healthy controls, who were aged between two and 10 years. The serum levels of hs-CRP and immunoglobulin (IgG, IgM, IgA) were examined by immunoturbidimetry and enzyme-linked immunosorbent assay (ELISA). Platelet indices were recorded. Descriptive statistics were calculated, and the t-test and correlation was utilized in univariate analysis; further logistic regression according to the enter rule was used for predictors of severe thrombocytopenia.

Results: Patients with ITP had markedly elevated hs-CRP and Ig profiles abnormalities than controls. There were lower platelet counts with higher CRP and lower IgA. The logistic regression identified hs-CRP and IgA as 2 independent risk factors for severe thrombocytopenia.

Conclusion: The CRP and immunoglobulin profiles could be of value in the understanding of the immunopathogenesis of pediatric ITP, and might serve as adjunctive markers in monitoring disease Non-Admitted subjects.